index

TripleTOF® Series

Fundamentals LC/MS Workflow:Can prepare standard samples and HPLC mobile phaseCan set up an LC/MS systemCan perform Compound Optimization on the Mass SpecCan create an LC/MS methodCan create and run a sample batchCan process data to obtain calibration curves and results using MultiQuant.Can distinguish between an application and LC/MS issueTools & Measurement:Demonstrates proper use of Hand ToolsDemonstrates proper use of Measurement ToolsDemonstrates proper use of Diagnostic ToolsDemonstrates proper use of Electrostatic Discharge KitDemonstrates safe use of tools and measurement equipmentTroubleshooting Skills:Understands the need to use a systematic troubleshooting processDemonstrates working knowledge of troubleshooting MS systemsCan interpret data and spectra results to facilitate troubleshooting processPossess the skill to distinguish between an application issue from a hardware issueGood Laboratory Practices:Complies with or oversees the compliance with Laboratory safety policies and procedures both at customers site and at SCIEX facilitiesTakes personal responsibility for safety Wears protective eye wear, gloves and where applicable lab coat and handling samplesUnderstands the proper use of labeling, storage and disposal of chemicals and solventsMakes sure instrument can be safely operatedMakes sure there is proper laboratory ventilation for the Mass Spec before installation and/or operationKeeps a safe working environment during a service activityKnows how to safely use test equipment with live instrumentation

Fundamentals of Instrumentation:Demonstrates understanding of the Mass Spec Hardware Components to a functional levelDemonstrates understanding of the theory and operations of Mass Spec (separation and focusing of Ions)Installation:Demonstrates understanding of Pre-Site requirementsDemonstrates understanding of Mass Spec Installation following the Installation ChecklistCan perform Customer FamiliarizationService & Maintenance Procedures:Demonstrates understanding of Mass Spec Maintenance following the Maintenance ChecklistCan perform Front End cleaning following Global Cleaning Procedure Wears protective eye wear, gloves and where applicable labcoat and handling samplesDemonstrates understanding of how to remove, disassemble, reassemble and reinstall instrument’s ion optics, detector etc.Tuning & Optimization:Independently perform ion source optimizationIndependently perform detector optimizationIndependently perform routine parameter optimization for all scan modes at Triple Quad functionsIndependently perform routine parameter optimization for all scan modes at Trap functionsIndependently perform mass calibrationHardware Fundamentals & Operation:Demonstrates understanding of the instrument’s electronic operation (i.e. which pcb controls a particular function, where to go for key measurements, which power supply provides power to a particular component etc.)Demonstrates understanding of the instrument´s vacuum components and operationCapable to relate parameters with their effect on performanceDemonstrates understanding of the instrument’s diagnostic software

IntroductionSciex product platforms overviewMarket overview (LCs + MSs Blue Line, Legacy TTOF)Customer Workflows Safety (EHS Training)Personal safety and the use of PPE (Personal protective equipment)Nitrile powder-free glovesLab coatSafety glassesElectrical safety (ESD)Good laboratory practicesLab safety and safety of othersComplying with Customer site requirements Installation / PM Computer and Mass Spectrometer preparationCommunication between PC and MSInstallation and updating of firmware and configuration filesSoftware installationAnalystMulti QuantStatus Scope

Program Description

API & QTRAP LEGACY PRODUCT WORKSHOP

Pre-PM Optimize Q1±, Q3± at 10Da/s (Ion source, Detector, Offsets, IE, DP) (Manually) Optimize MS2 (Manually)

Day 4

The 3500/4500/5500/6500 series Training Program covers parts of installation, maintenance, troubleshooting and repair. The course will encompass theory of operation of Mass Spectrometer and hardware. The FSE performs several exercises to develop the skills of servicing SCIEX products. The fundamentals of troubleshooting the LC/MS system using diagnostic tools will also be covered. Out of scope tasks It is important to understand that the FSE is not fully certified to service all Sciex Mass Spectrometers. This training includes only 3500/4500/5500/6500 series instruments. This training does not certify the FSE to work on Medical Device (MD) systems. The 3200/4000/5000 (Legacy) series instruments will not be covered in this course. This course will not include the installation and servicing of HPLC devices.

Fundamentals of LC/MSHPLC introduction Components and Functions Connections and Communication Purging Sample preparation / mobile phase Compound optimization (automatic with syringe) Source optimization (manual, pump flow+ syringe) Building and Editing method & batch Run Triazine measurements with column

Day 5

Agenda

SelexIon For TripleTOF – Module 3: Webex

Data analysis using Multi Quant and Analyst PM related activities Vacuum Test Source Front-end cleaning Filter cleaning

Installation verification Tuning and Optimization of all Quad scan speeds (manually) Tuning and Optimization of Trap modes with instrument optimization function

Competency

Training Program

TroubleshootingTroubleshooting Process for complete system (Customer, Computer, Software, HPLC, MS) Exercises with TEAMS How to use TEAMS Troubleshoot MS with TEAMS Vacuum Low Signal Communication Exercises with identifying root causes of complete platform because of data/chromatograms Unstable Signals Low Signals Retention Time Shift

Learner Description

• Pre-PM - Optimize Q1±, Q3± at 10Da/s (Ion source, Detector, Offsets, IE, DP) (Manually) - Optimize MS2 (Manually) • Triazine LC/MS/MS Workflow - HPLC introduction • Components and Functions • Connections and Communication • Purging - Sample preparation / mobile phase - Compound optimization (automatic with syringe) - Source optimization (manual, pump flow+ syringe) - Building and Editing method & batch - Run Triazine measurements with column - Data analysis using Multi Quant and Analyst • PM related activities - Vacuum Test - Source - Front-end cleaning - Filter cleaning - Additional: • Rail pull • Global Cleaning Procedure (Alconox and new) • Installation verification - Tuning and Optimization of all Quad scan speeds (manually) - Testing, Tuning and Optimization of Trap modes with instrument optimization function - Source Test • Review CFC documentation IQ/OQ • Review IQ Documentation • Review OQ/IPV Documentation Service procedures • Testing the Mass Spectrometer (ASD, Tuning Tools) • Parts replacement Troubleshooting • Troubleshooting Process for complete system (Customer, Computer, Software, HPLC, MS) • Exercises with TEAMS - How to use TEAMS - Troubleshoot MS with TEAMS • Vacuum • Low Signal • Communication • Exercises with identifying root causes of complete platform because of data/chromatograms - Unstable Signals - Low Signals - Retention Time Shift Participants• Maximum 6 FSE.

Additional: Rail pull Global Cleaning Procedure (Alconox and new)

Day 6

The evaluation for the associate’s training completion will be based on the following competencies:

Installation verification Tuning and Optimization of all Quad scan speeds (manually) Tuning and Optimization of Trap modes with instrument optimization function Source Test

This training program will assume the trainee has received pre-class mentoring. The learner will be required to meet with the instructor in a pre-class conference call (four weeks before class) to identify and address knowledge gaps and to determine the best way to prepare for class. A minimum of two weeks prior to attending the course, the learner will have completed the pre-requisite learning and the pre-mentoring checklist. This checklist will be reviewed during that call. It is expected that the learner has the following skills: Fundamental knowledge of LCMSFundamental knowledge of chemistrySome field service experienceSome laboratory experience and knowledge of laboratory safetyGood problem solving abilitiesGood communication skillsThis training is not for Service Technicians. Learner ResponsibilitiesCompletes all assigned training tasksDevelops knowledge of SCIEX productsLearns installation of BlueLine instruments and related model differencesRemains actively engaged and participates in all class and lab activitiesLearns and develops the technical skills necessary to perform quality maintenanceLearns and develops the skills to support customer LC/MS workflow

Day 10

Day 1

Prerequisites: • Pre-Mentoring Checklist • Interview with instructor (4 weeks before training) • Completion of assigned pre-learnings Pre-Learning: • Special assigned ELearning on MyDevelopment (4 weeks) • Installing & Using the Offline Player for eLearning • SharePoint Access to service databases, ePics, etc. • Safe lifting • ESD training • Blueprint for success Instructor Led Training (ILT):Soft Skills • Blueprint for Success - Acquire and practice soft skills necessary to interact with customers • Setting customers’ expectations. • FSE needs to effectively communicate to costumer. Instruments covered • Mass spectrometry - 3500 (API) - 4500 (API & Qtrap) - 5500 (API & Qtrap) - 6500/6500+ (API & Qtrap) - Liquid Chromatography Introduction • Sciex product platforms overview • Market overview (LCs + MSs Blue Line, Legacy TTOF) • Customer Workflows Safety (EHS Training) • Personal safety and the use of PPE (Personal protective equipment) - Nitrile powder-free gloves - Lab coat - Safety glasses • Electrical safety (ESD) • Good laboratory practices - Lab safety and safety of others - Complying with Customer site requirements Installation / PM • Computer and Mass Spectrometer preparation - Communication between PC and MS - Installation and updating of firmware and configuration files - Software installation • Analyst • Multi Quant • Status Scope

Day 7

Day 2

Day 3

Review CFC documentation IQ/OQ Review IQ Documentation Review OQ/IPV Documentation

Day 8

X500 QTOF System

Service proceduresTesting the Mass Spectrometer (ASD, Tuning Tools) Parts replacement

SelexION Technology – Module 3: Webex

Day 9

3500/4500/5500/6500 Series LC/MS System

The trainee will have completed Blueline training. It is expected that the learner has the following skills: Fundamental knowledge of LCMSFundamental knowledge of chemistrySome field service experienceSome laboratory experience and knowledge of laboratory safetyGood problem solving abilitiesGood communication skillsThis training is not for Service Technicians. Learner ResponsibilitiesCompletes all assigned training tasksDevelops knowledge of SCIEX productsLearns installation of Legacy instruments and related model differencesRemains actively engaged and participates in all class and lab activitiesLearns and develops the technical skills necessary to perform quality maintenanceLearns and develops the skills to support customer LC/MS workflow

Overview of differences in Legacy optics/tuning Instrument pre-PM checks Workbook Chapter 1/2/3 - Preparing for Maintenance - Removing ion optics assy - Disassembly of mass rail - Reassembly of mass rail - Continuity/resistance checks - Refit ion optics assy - Pump down - Soft start

Legacy products • Installation - MS setup - Connecting gases, electrical - Roughing pump setup - Computer setup + cards - Installing Analyst, drivers, instrument files - Pump down sequence - Proper system venting procedure - Installation specifications - Quad tuning - Manual trap tuning• PM - PM - Front end cleaning - Voltage checks - Coil box tuning - Filter replacement - Oil replacement• Repair - Module function & replacement - Rail Pull - Rail disassembly/reassembly - Rail continuity/resistance checks• LCMS Triazine Workflow - Sample prep - Compound optimization - Creating methods & batch - Quantitation using Analyst quant/Multiquant Participants• Maximum 6 FSEs.

The evaluation for the associate’s training completion will be based on the following competencies:

Workbook Chapter 4 - Voltage checks - Lens calibration - RF coilbox tuning - Performance RF tuning - CAD gas optimization Post PM Checks/Tuning

MSMSMS Tuning - Englebert Script

Prerequisites: • Blueline trained Pre-Learning: • None Instructor Led Training (ILT): Instruments covered • Mass spectrometry - 3200 (API & Qtrap) - 4000 (API & Qtrap) - 5000 (API) Safety (EHS Training) • Personal safety and the use of PPE (Personal protective equipment) - Nitrile powder-free gloves - Lab coat - Safety glasses • Electrical safety (ESD) • Good laboratory practices - Lab safety and safety of others - Complying with Customer site requirements Basic Electronics • Electrical Safety • Using a DVM • Using a HV Probe Service Tools• Analyst Service Diagnostics (ASD) - Updating firmware and config type files - Readbacks - Module status

The API & QTrap Legacy Product Workshop covers parts of installation, maintenance, troubleshooting and repair. The course will cover the differences in the ion optics and tuning of Legacy instruments compared to Blueline. The FSE performs several exercises to develop the skills of servicing SCIEX products. The fundamentals of troubleshooting the LC/MS system using diagnostic tools will also be covered. Out of scope tasks It is important to understand that the FSE is not fully certified to service all Sciex Mass Spectrometers. This training includes only Legacy instruments. This training does not certify the FSE to work on Medical Device (MD) systems. This course will not include the installation and servicing of HPLC devices.

Workbook Chapter 6 - Hardware identification - About instrument controller and communication - About ion source LCMS Triazine Workflow

Workbook Chapter 5 - High Mass PPG Calibration - Enh Res - C2B optimization - EMC

LAB:Install NanoSprayIII Prepare Test Standards GluFib Test NSIII

The TripleTOF Service Training program consists of e-learning and instructor-led training. Some e-learning assignments must be completed by the trainee before they can attend the class. Other e-learning modules are reviewed as part of the class or will need to be completed after attending the class. The Training Program covers technology concepts, site installation inspection, installation, testing, maintenance, troubleshooting and repair of SCIEX TripleTOF systems at customer sites. The training will also provide basic knowledge and practice on the Triazine sample workflow and PeakView software required to view the data. After the training the Field Service Engineer will know the different TripleTOF systems and associated peripherals, and will be able to visit customer sites to perform site inspections, installations, IQOQ validation, and planned maintenance of TripleTOF instruments. They will also be able to perform troubleshooting and repair of the TripleTOF systems as well some workflow support including installation of data processing computers and add-on software. Out of scope tasks Advanced application support:This training program does not prepare the FSE to perform advanced application support that requires specialized knowledge of the customer’s samples, sample preparation techniques, workflows and software functionality. Computer networking:This training program does not prepare the FSE to perform advanced computer support that requires specialized knowledge of IT systems, networks, computers, and software. Basic TripleQuad training:The trainee should have knowledge of concepts such as ionization techniques; quadrupole ion transmission and filtering theory; interface and mass rail cleaning; planned maintenance; IQOQ validation. This knowledge should have been acquired through training and field service assignments on SCIEX TripleQuad and QTRAP instrumentation. Associated tasks and concepts are only practiced or discussed in this training as necessary to cover the differences for TripleTOF. Ion Sources:Not covered are the TurboV source, PhotoSpray source, IonDrive source, SelexION, and 3rd party ion sources such as Advion. Basic Analyst software training:Basic Analyst software training is not covered. The trainee should have knowledge of concepts such as Analyst software and PC card installation, and detailed understanding of the parameter functionality in the Parameter and Instrument Data tables. Eksigent nanoLC systems:This training covers the installation and testing of the NanoSpray III and PicoView sources. It does not cover the installation, maintenance, troubleshooting and repair of nanoLC systems, which requires in-depth training and practice. This is covered in a different training program. Troubleshooting using an oscilloscope:An oscilloscope is used in this training to demonstrate several TripleTOF concepts. This is for clarification purposes only. Knowledge of troubleshooting the TripleTOF systems with an oscilloscope is not required and therefore not covered in this training. Capillary Electrophoresis (CE):This training does not cover the connection and operation of CE systems to the TripleTOF.

CLASS:Calibration of the TripleTOF® Series LAB:Setting up CDS LAB:Triazine workflow

CLASS:Detailed System hardware and operation. Voltages that Affect Peak Shape and Mass Calibration Time To Digital Converter Enhance Mode LAB: Measure Electronic Signals - Card Cage Test Points (DVM) - DCA/DCB in resolving/non-resolving mode (DVM) - Q1/Q2 RF in the 4 scan modes (scope) - IQ0 pulsing (scope demo) - IQ3 pulsing (scope demo) - Pulser module (scope demo)

Prerequisite Training Programs• Legacy API & QTRAP Training Program or• 4500/5500/6500 Series LC/MS Systems Certification Program• Training on at least one type of SCIEX-supported LC system Note: Before attending the training program, the trainee should have 6 to 12 months of extensive field service experience on TripleQuad, QTRAP, and HPLC products. ELearning• Triazine IDA Workflow• TripleTOF Theory of Operation • TripleTOF Uncrating New Greenpac Style Crate • TripleTOF Hardware Installation• TripleTOF 4600 Hardware and Functionality• TripleTOF 6600 Hardware and Functionality• TripleTOF Ion Optics Voltages (6 modules) • TripleTOF Reflector Removal and Replacement • TripleTOF MCP Detector Assembly Removal and Replacement • TripleTOF Accelerator Removal and Replacement • TripleTOF Pulser Module Testing • TripleTOF MCP Detector Repair Procedure • TripleTOF 5600 How to Service the Ion Optics Rail • TripleTOF 6600 How to Service the Ion Optics Rail Instructor Led Training (ILT)Instruments covered• Mass spectrometry - TripleTOF 4600 - TripleTOF 5600 - TripleTOF 6600• Ion Sources - DuoSpray source - NanoSpray III source installation• Workflows - Triazine IDA Workflow• Software - Analyst TF - PeakView Service Tools• Analyst Service Diagnostics (ASD)• DVM FLUKE 187, 189, 287, or 289 (This model is required to work on TripleTOF systems)• Fluke High Voltage Probe 40 kV• Service Tools kit (PN 1039438)• Access to the SCIEX Ion Path Cleaning Kit Reviewing the Basics• Concepts of HPLC and nanoLC in conjunction with TripleTOF systems.• Concept of the Triazine workflow on TripleTOF.• TripleTOF concept of operation. Safety• Electrical safety awareness when working around high voltages• Overview and location of dangerous TripleTOF voltages Good laboratory practices• Lab safety and safety of others• Personal safety and the use of PPE (Personal protective equipment) - Nitrile powder-free gloves - Lab coat - Safety glasses• Complying with Service Training site requirements Basic Electronics• Using the FLUKE 289 DVM• Using a High Voltage Probe for ‘low voltage - low load’ measurements Troubleshooting• Troubleshooting the installation - Pump down sequence • Common vacuum problems • Pump down not starting - Acceptance Testing • Sensitivity issues • Resolution issues • Mass drift• Common TripleTOF problems and how to troubleshoot Customer Familiarization• Triazine workflow using IDA in combination with CDS• Use of PeakView software to review data Introduction• Presentation TTOF compared with QQQ• Review of pre-learning• Basic Technology Concept - QQTOF principle

CLASS:TripleTOF Concept Explanation of Instrument Parameters LAB: Lens Calibration Wizard - ASD - Tools - Safety CLASS:Standards Kit Site Acceptance Tests Interpretation of TOF data

CLASS:TTOF compared with QQQ CLASS:Review of pre-learning CLASS:Site preparation (discussion) LAB:Hardware identification − Vacuum System − QUAD / TOF − Power Supplies − Electronics Modules CLASS:TripleTOF Demo - Mass Standards Kit - Instrument Performance - QUAD Scan - MS TOF Scan - MS/MS TOF Scan

CLASS:Troubleshooting and Service Procedures LAB:− Pulser Module − Accelerator − Detector − MCP Replacement − TOF Entrance Lens − Reflector − 5600 Ion Optics − 6600 Ion Optics

LAB:TEAM 1: Tuning and Testing Installation 5600 LAB:TEAM 2: Tuning and Testing Installation 6600 LAB:TEAM 1 & 2 switch instruments and complete pos mode only

- Scan modes - Instrument Parameters - ITC - Quad - TOF - Enhanced mode• Vacuum System• QUAD / TOF• Power Supplies• Electronics Modules Installation• Instrument setup• Pumpdown• Mass Standards Kit• Instrument Performance• QUAD Scan• MS TOF Scan• MS/MS TOF Scan• Lens Calibration Wizard - ASD - Tools - Safety• Interpretation of TOF data - Peak Height - Peak Area - Centroid Intensity - Sum Intensity• Calibration of the TripleTOF® Series• Setting up CDS Service Procedures• Test the Pulser Module• Remove and Replace the Accelerator Assembly• Remove and Replace the Detector Assembly• The TripleTOF® Series MCP Replacement Procedure• Remove/replace/Clean TOF Entrance Lens• Remove and Replace the Reflector Assembly• TripleTOF®5600 – How to Service the Ion Optics Rail• TripleTOF®6600 – How to Service the Ion Optics Rail Troubleshooting• Voltages in the Mass Rail - TOF Entrance Lens Voltages - Voltages that Affect Peak Shape and Mass Calibration• Time To Digital Converter• Ion Transfer in the Mass Rail vs TOF• Enhance Mode• Measure Electronic Signals - Card Cage Test Points (DVM) - DCA/DCB in resolving/non-resolving mode (DVM) - Q1/Q2 RF in the 4 scan modes (scope demo) - IQ0 pulsing (scope demo) - IQ3 pulsing (scope demo) - Pulser module (scope demo) NanoSpray• Installation of the interface and source• Hardware Components• Sprayer Rail Assembly• Prepare Test Standards - [Glu1]-Fibrinopeptide B• Test NanoSpray®III - MS TOF - MS/MS TOF - Instrument Optimization using NanoSpray Participants• Maximum 2 trainees per available instrument. The number of available instruments depends on the training location. Pre-Work• Basics of workflows: - Triazine IDA Workflow • TripleTOF Theory of Operation • TripleTOF Uncrating New Greenpac Style Crate • TripleTOF Hardware Installation

The training program will assume the trainee matches the following profile and does not have TripleTOF or X500 Series experience. Detailed knowledge of the concept, hardware, operation and use of SCIEX TripleQuad and QTRAP systems.Extensive field service experience in the troubleshooting and maintenance of mass spectrometersHas field service experience in the installation and operation of Analyst software.Has field service experience in the installation and troubleshooting of Data Acquisition computers.Has field service experience in installation and operation of at least one type of SCIEX-supported LC system.Has field service experience in the use of diagnostic tools for testing and troubleshooting purposes including ASD, TEAMS, and a Digital Voltmeter.Must know the basic steps of sample and solvent preparation.Is trained on Lab SafetyIs trained on Electrical SafetyIs able to learn the advanced technology concepts associated with hybrid mass spectrometers such as TripleTOF.Is able to learn troubleshooting and problem solving procedures related to TripleTOF systems.Is able to learn the general knowledge required to understand basic workflows associated with TripleTOF from sample preparation to data interpretation.Job ResponsibilitiesIn this advanced-level position, the FSE works independently on TripleTOF systems and is able to do site installation inspection, installation, maintenance, and repair of SCIEX TripleTOF systems at customer sites. The FSE also supports customers with basic workflows and software knowledge associated with TripleTOF. The TripleTOF trained FSE may need some mentoring from experienced TripleTOF trained FSEs before they perform service tasks independently. Learner’s ResponsibilitiesCompletes all assigned training tasks.Remains actively engaged and participates in all class and lab activities.Develops adequate knowledge to successfully troubleshoot SCIEX TripleTOF products Learns and develops the technical skills necessary to perform high quality maintenance and repair on SCIEX TripleTOF products.Learns and develops the skills to support the customer LC/MS workflow.

LAB:TEAM 1: Tuning and Testing Installation 5600 LAB:TEAM 2: Tuning and Testing Installation 6600

Safety • Electrical safety awareness when working around high voltages• Overview and location of dangerous X500 series voltages Good laboratory practices• Lab safety and safety of others• Personal safety and the use of PPE (Personal protective equipment) - Nitrile powder-free gloves - Lab coat - Safety glasses• Complying with Service Training site requirements Basic Electronics • Using the FLUKE 289 DVM • Using a High Voltage Probe for ‘low voltage - low load’ measurements Troubleshooting• Troubleshooting the installation - Pump down sequence • Common vacuum problems • Pump down not starting - Acceptance Testing • Sensitivity issues • Resolution issues • Mass drift - Common problems and how to troubleshoot Participants• Maximum 2 trainees per available instrument. The number of available instruments depends on the training location. Pre-Work• General knowledge to understand workflows• Basics of workflows: - Triazine IDA Workflow - Workflow using SWATH - Workflow using MultiQuant• The X500 series elearning modules are in the development stage - X500 QTOF Series Theory of Operation - X500 QTOF Series Uncrating the Instrument - X500 QTOF Series Hardware Installation - X500 QTOF Series Hardware Walk Around

The training program will assume the trainee matches the following profile and does not have TripleTOF. Detailed knowledge of the concept, hardware, operation and use of SCIEX TripleQuad and QTRAP systems.Extensive field service experience in the troubleshooting and maintenance of mass spectrometersHas field service experience in the installation and operation of Analyst software.Has field service experience in the installation and troubleshooting of Data Acquisition computers.Has field service experience in installation and operation of at least one type of SCIEX-supported LC system.Has field service experience in the use of diagnostic tools for testing and troubleshooting purposes including ASD, TEAMS, and a Digital Voltmeter.Must know the basic steps of sample and solvent preparation.Is trained on Lab SafetyIs trained on Electrical SafetyIs able to learn the advanced technology concepts associated with hybrid mass spectrometers such as the QTOF series.Is able to learn troubleshooting and problem solving procedures related to QTOF systems.Is able to learn the general knowledge required to understand basic workflows associated with QTOF from sample preparation to data interpretation.Job ResponsibilitiesThis advanced-level position requires the FSE to work independently on X500 series systems and be able to do site installation inspection, installation, maintenance, and repair of SCIEX X500 series systems at customer sites. The FSE also supports customers with basic workflows and software knowledge associated with the X500 series. The X500 series trained FSE may need some mentoring from experienced X500 series trained FSEs before they perform all service tasks independently. Learner’s Responsibilities Completes all assigned training tasks.Remains actively engaged and participates in all class and lab activities.Develops adequate knowledge to successfully troubleshoot SCIEX X500 series products Learns and develops the technical skills necessary to perform high quality maintenance and repair on SCIEX X500 series products.Learns and develops the skills to support the customer LC/MS workflow.

Lab:TEAM 1: Tuning and Testing Installation X500R TEAM 2: Tuning and Testing Installation X500B

− Test the Pulser Module − Remove and Replace the Accelerator Assembly − Remove and Replace the Detector Assembly − The X500 Series MCP Replacement Procedure - Remove/Clean the Transfer Optics − Remove and Replace the Reflector Assemblies − X500 Series – How to Service the QUAD Optics − X500 Series – How to Service the TOF Optics - How to Service the Transfer Optics

Triazine workflow using IDA in combination with CDS Presentation X500 compared with QQQ Review of pre-learning Lab: Hardware Walk Around − Vacuum System − QUAD / TOF − Power Supplies − Electronics Modules Lab: Tuning Tools and SCIEX OS Software Demo - Mass Standards Kit - Instrument Performance - QUAD Scan - MS TOF Scan - MS/MS TOF Scan

The X500 Series Service Training program consists of e-learning and instructor-led training. Some e-learning assignments must be completed by the trainee before they can attend the class. Other e-learning modules are reviewed as part of the class or will need to be completed after attending the class. The Training Program covers technology concepts, site installation inspection, installation, testing, maintenance, troubleshooting and repair of SCIEX X500 series systems at customer sites. The training will also provide basic knowledge and practice on sample workflows and software required to run samples and process data. After the training the Field Service Engineer will know the different X500 series systems and associated peripherals, and will be able to visit customer sites to perform site inspections, installations, IQOQ validation, and planned maintenance of X500 series instruments. They will also be able to perform troubleshooting and repair of the X500 series systems as well as basic software and workflow support including installation of data processing computers and add-on software. Out of scope tasks Advanced application support:This training program does not prepare the FSE to perform advanced application support that requires specialized knowledge of the customer’s samples, sample preparation techniques, workflows and software functionality. Computer networking:This training program does not prepare the FSE to perform advanced computer support that requires specialized knowledge of IT systems, networks, computers, and software. Basic TripleQuad training:The trainee should have knowledge of concepts such as ionization techniques; quadrupole ion transmission and filtering theory; interface and mass rail cleaning; planned maintenance; IQOQ validation. This knowledge should have been acquired through training and field service assignments on SCIEX TripleQuad and QTRAP instrumentation. Associated tasks and concepts are only practiced or discussed in this training as necessary to cover the differences for TripleTOF. Ion Sources:Not covered are the TurboV source, PhotoSpray source, IonDrive source, SelexION, and 3rd party ion sources such as Advion. Basic Analyst software training:Basic Analyst software training is not covered. The trainee should have knowledge of concepts such as Analyst software and PC card installation, and detailed understanding of the parameter functionality in the Parameter and Instrument Data tables. Eksigent nanoLC systems:This training covers the installation and testing of the NanoSpray III and PicoView sources. It does not cover the installation, maintenance, troubleshooting and repair of nanoLC systems, which requires in-depth training and practice. This is covered in a different training program. Troubleshooting using an oscilloscope:An oscilloscope is used in this training to demonstrate several TripleTOF concepts. This is for clarification purposes only. Knowledge of troubleshooting the TripleTOF systems with an oscilloscope is not required and therefore not covered in this training.

Lab:TEAM 1: Tuning and Testing Installation X500R TEAM 2: Tuning and Testing Installation X500B

SelexIon For TripleTOF – Module 3: Webex

Wrap up Clean up Final exam

Voltages in the Mass Rail − Transfer Optics Voltages − Voltages that Affect Peak Shape and Mass Calibration − Time To Digital Converter (TDC) − Ion Transfer in the Mass Rail vs TOF Lab: Measure Electronic Signals - Test Points (DVM) - DCA/DCB in resolving/non-resolving mode (DVM) - Q1/Q2 RF in the 4 scan modes (scope) - IQ0 pulsing (scope demo) - Pulser module (scope demo)

Presentation Basic Theory Explanation of Instrument Parameters - Quad - TOF Tuning/Testing: • Tuning Tools Diagnostic Software • Standards Kit • Interpretation of TOF data - Peak Height - Peak Area - Centroid Intensity - Sum Intensity

Calibration of the X500 QTOF Series Lab: Setting up CDS Lab:Customer Familiarization Workflow − X500 Series QTOF Introduction to LC MSMS - Triazine CFC

Prerequisites: TripleTOF trainedPre-Learning SelexION Technology TheorySelexION Technology Hardware install for TripleTOFWebEx Session Instruments coveredSelexION on TripleTOFService ToolsAnalyst Service Diagnostics (ASD)Updating firmware and config type filesReadbacksModule statusSelexIon TechnologyTheoryIMSDMSSeparation Voltage (SV)Compensation Voltage (CV)ModifierBufferNew Parts at SelexIONFunction of new parts at SelexIONWhat is new in AnalystSVCVModifierBuffer gasSelexIon Installation Testo Run Installation tests with SelexIon mixo Practice functions in AnalystTroubleshootingCoil DippingNew CoilsOld CoilsDischargeDifferences at SelexION+New lensVoltage for Lens

The training program will assume the trainee matches the following profile: Knowledge of the concept, hardware, and operation of SCIEX TripleTOF systems.Extensive field service experience in the troubleshooting and maintenance of mass spectrometersHas field service experience in the installation and operation of AnalystTF software.Has field service experience in the installation and troubleshooting of Data Acquisition computers.Has field service experience in the use of diagnostic tools for testing and troubleshooting purposes including ASD and a Digital Voltmeter.Must know the basic steps of sample and solvent preparation.Is trained on Lab SafetyIs trained on Electrical SafetyIs able to learn the technology concept associated with SelexionIs able to learn troubleshooting and problem solving procedures related to Selexion systemsIs able to learn the general knowledge required to understand basic workflows associated with TripleTOF and Selexion from sample preparation to data interpretation.This training is only for FSEs who were previously trained on TripleTOF. Learner ResponsibilitiesCompletes all assigned training tasks.Remains actively engaged and participates in all class and lab activities.Develops adequate knowledge to successfully troubleshoot SCIEX Selexion on TripleTOF products Learns and develops the technical skills necessary to perform high quality maintenance and repair on SCIEX Selexion products.

The SelexIon Technology Training covers parts of installation, maintenance, troubleshooting and repair. The course will cover the theory of DMS, new parts and workflows of SelexIon. The FSE performs several Installation Tests. The fundamentals of troubleshooting the SelexIon system will also be covered. Out of scope tasks It is important to understand that the training will not cover TripleTOF mass spectrometers. This training includes only SelexIon Technology for TripleTOF instruments.

The trainee will have completed Blueline training. It is expected that the learner has the following skills: Fundamental knowledge of LCMSFundamental knowledge of chemistrySome field service experienceSome laboratory experience and knowledge of laboratory safetyGood problem solving abilitiesGood communication skillsThis training is not for Service Technicians. Learner ResponsibilitiesCompletes all assigned training tasksDevelops knowledge of SCIEX productsLearns installation of SelexIon on BlueLine instrumentsLearns and develops the technical skillsLearns and develops the skills to support customer LC/MS workflow

Prerequisites: • Blueline trained Pre-Learning: • SelexIon 5500 Installation• SelexIon 5500 Theory• Installation SelexIon Technology on 500 System WebEx Session: Instruments covered• SelexION Service Tools• Analyst Service Diagnostics (ASD) - Updating firmware and config type files - Readbacks - Module status SelexIon Technology• Theory - IMS - DMS - Separation Voltage (SV) - Compensation Voltage (CV) - Modifier - Buffer - New Parts at SelexION - Function of new parts at SelexION - What is new in Analyst • SV • CV • Modifier • Buffer gas • SelexIon Installation Test - Run Installation tests with SelexIon mix - Practice functions in Analyst • Troubleshooting - Coil Dipping • New Coils • Old Coils - Discharge • Differences at SelexION+ - New lens - Voltage for Lens Participants: • Maximum 2 FSEs.

The SelexIon Technology Training covers parts of installation, maintenance, troubleshooting and repair. The course will cover the theory of DMS, new parts and workflows of SelexIon. The FSE performs several Installation Tests. The fundamentals of troubleshooting the SelexIon system will also be covered. Out of scope tasks It is important to understand that the FSE is not fully certified to service all Sciex Mass Spectrometers. This training includes only SelexIon Technology for BlueLine instruments. This training does not certify the FSE to work on Medical Device (MD) systems.This course will not include the installation and servicing of HPLC devices.